Investigation of α-Synuclein Fibril Structure by Site-directed Spin Labeling

Structure of membrane-bound -synuclein studied by site-directed spin labeling

Many of the proposed physiological functions of -synuclein, a protein involved in the pathogenesis of Parkinson’s disease, are related to its ability to interact with phospholipids. To better understand the conformational changes that occur upon membrane binding of monomeric -synuclein, we performed EPR analysis of 47 singly labeled -synuclein derivatives. We show that membrane interaction is m...

Structure of membrane-bound alpha-synuclein from site-directed spin labeling and computational refinement.

alpha-Synuclein is known to play a causative role in Parkinson disease. Although its physiological functions are not fully understood, alpha-synuclein has been shown to interact with synaptic vesicles and modulate neurotransmitter release. However, the structure of its physiologically relevant membrane-bound state remains unknown. Here we developed a site-directed spin labeling and EPR-based ap...

Structure of the inhibitory region of troponin by site directed spin labeling electron paramagnetic resonance.

Site-directed spin labeling EPR (SDSL-EPR) was used to determine the structure of the inhibitory region of TnI in the intact cardiac troponin ternary complex. Maeda and collaborators have modeled the inhibitory region of TnI (skeletal 96-112: the structural motif that communicates the Ca(2+) signal to actin) as a kinked alpha-helix [Vassylyev, D., Takeda, S., Wakatsuki, S., Maeda, K. & Maeda, Y...

Membrane Protein Structure and Dynamics Studied by Site-directed Spin Labeling Esr

Noncovalent and site-directed spin labeling of nucleic acids.

Electron paramagnetic resonance (EPR) spectroscopy is widely used to study free radicals or paramagnetic centers associated with biopolymers. With the advent of pulsed EPR methods, which allow accurate distance measurements between 20 and 80 , structures of biopolymers have increasingly been interrogated by this technique. Some of the advantages of EPR spectroscopy over other structural techniq...